Table 2. Sequence of primers used in two-step RT-qPCR [27]

Primer	Sequence (5’-3’)	Amplified gene	Size (bp)	Tm (°C)
ldh-F	TTGGCGACGCTCTTGATCTTAG	Lactat dehydrogenase (ldh)	92	62.1
ldh-R	GTCAGCATCCGCACAGTCTTC			63.3
gtfB-F	AGCAATGCAGCCATCTACAAAT	Glucosyltransferase–I (gtfB)	98	58.4
gtfB-R	ACGAACTTTGCCGTTATTGTCA			58.4
luxS-F	ACTGTTCCCCTTTTGGCTGTC	S- ribosylhomocystein lyase (luxS)	93	61.3
luxS-R	AACTTGCTTTGATGACTGTGGC			60.3