Table 2. Recovery of MTX (n=5)

Concentrations of QC samples (μg/mL) Sample number Found concentrations (μg/mL) Recovery (%) Mean ± SD (%)
0.5 1 0.34 68.76 70.08 ± 1.41
2 0.36 72.03
3 0.35 69.44
4 0.35 69.09
5 0.36 71.07
10 1 8.51 85.07 81.99 ± 3.30
2 8.28 82.84
3 7.81 78.05
4 8.50 84.96
5 7.90 79.01
20 1 15.86 79.28 80.19 ± 2.51
2 16.70 83.51
3 15.56 77.79
4 15.65 78.24
5 16.43 82.14