Selectivity | Analyte (% LLOQ) | - | 0.00 | 0.00 | |
Internal standard (% IS) | - | 0.00 | 0.00 |
Carryover | Analyte (% LLOQ) | - | 0.00 | 0.00 | 0.00 |
Internal standard (% IS) | - | 0.00 | 0.00 |
Linearity | R square (R2) | - | 0.9984 | 0.9988 | |
Intra-day accuracy and precision | Accuracy (%) | LLOQ | 102.64 | 95.68 | |
LQC | 107.15 | 87.56 | |
MQC | 107.67 | 104.38 | |
HQC | 114.13 | 109.54 | |
| Precision, CV (%) | LLOQ | 9.18 | 12.40 | |
LQC | 5.18 | 8.47 | |
MQC | 3.50 | 7.39 | |
HQC | 3.69 | 6.46 | |
Inter-day accuracy and precision | Accuracy (%) | LLOQ | 102.16 | 99.54 | |
LQC | 105.22 | 94.58 | |
MQC | 103.19 | 100.15 | |
HQC | 107.08 | 104.10 | |
| Precision, CV (%) | LLOQ | 11.16 | 12.40 | |
LQC | 6.29 | 8.47 | |
MQC | 6.20 | 7.39 | |
HQC | 6.90 | 6.46 | |
Matrix effect | Precision, CV (%) | LQC | 3.47 | 4.44 | |
HQC | 1.94 | 0.78 | |
Recovery | Mean | LQC | 89.51 | 84.01 | 84.08 |
MQC | 83.61 | 92.46 | 82.36 |
HQC | 81.41 | 88.86 | 76.46 |
| Precision, CV (%) | LQC | 2.50 | 3.66 | 2.74 |
MQC | 2.57 | 4.39 | 4.68 |
HQC | 2.05 | 2.95 | 3.89 |
Dilution integrity | Accuracy (%) | - | 97.52 | 100.69 | |
Precision, CV (%) | - | 2.78 | 4.62 |
Free-thaw stability | % of nominal concentration | LQC | 98.56 | 97.18 | |
HQC | 107.64 | 99.88 |
At room temperature for 6 hours | % of nominal concentration | LQC | 110.42 | 90.83 | |
HQC | 93.51 | 104.27 |
In autosampler for 40 hours at 4°C | % of nominal concentration | LQC | 107.86 | 90.40 | |
HQC | 105.76 | 105.02 |
Long-term stability (after 95 days) | % of nominal concentration | LQC | 92.59 | 91.00 | |
HQC | 104.44 | 105.61 |