Table 2. Summary of validation results

Property QC SIM SIM-A IS
Selectivity Analyte (% LLOQ) - 0.00 0.00
Internal standard (% IS) - 0.00 0.00
Carryover Analyte (% LLOQ) - 0.00 0.00 0.00
Internal standard (% IS) - 0.00 0.00
Linearity R square (R2) - 0.9984 0.9988
Intra-day accuracy and precision Accuracy (%) LLOQ 102.64 95.68
LQC 107.15 87.56
MQC 107.67 104.38
HQC 114.13 109.54
Precision, CV (%) LLOQ 9.18 12.40
LQC 5.18 8.47
MQC 3.50 7.39
HQC 3.69 6.46
Inter-day accuracy and precision Accuracy (%) LLOQ 102.16 99.54
LQC 105.22 94.58
MQC 103.19 100.15
HQC 107.08 104.10
Precision, CV (%) LLOQ 11.16 12.40
LQC 6.29 8.47
MQC 6.20 7.39
HQC 6.90 6.46
Matrix effect Precision, CV (%) LQC 3.47 4.44
HQC 1.94 0.78
Recovery Mean LQC 89.51 84.01 84.08
MQC 83.61 92.46 82.36
HQC 81.41 88.86 76.46
Precision, CV (%) LQC 2.50 3.66 2.74
MQC 2.57 4.39 4.68
HQC 2.05 2.95 3.89
Dilution integrity Accuracy (%) - 97.52 100.69
Precision, CV (%) - 2.78 4.62
Free-thaw stability % of nominal concentration LQC 98.56 97.18
HQC 107.64 99.88
At room temperature for 6 hours % of nominal concentration LQC 110.42 90.83
HQC 93.51 104.27
In autosampler for 40 hours at 4°C % of nominal concentration LQC 107.86 90.40
HQC 105.76 105.02
Long-term stability (after 95 days) % of nominal concentration LQC 92.59 91.00
HQC 104.44 105.61