Table 15. Optimum freeze-drying media for preservation of Lactobacillus strains

Lactobacilli	Optimal freeze-drying formulation	% survival	Ref
L. acidophilus (NCDC 291)	1.2% Sucrose + 6% skim milk	69.86	[27]
L. acidophilus (NCDC 015)		68.84
L. acidophilus (MTCC 10307)		67.92
L. lactis Sr. 3.54	10% (Sucrose + skim milk)	62.0	[28]
L. paracasei subsp. tolerance (DSM 20258)	6% Skim milk + 8% Trehalose + 4% sodium ascorbate	82.0	[29]
L. delbrueckii subsp. bulgaricus (DSM 20081)	6% Skim milk + 8% Trehalose + 4% sodium ascorbate	74.0	[29]
L. plantarum JH287	10% Skim milk + 10% Sucrose	77.15	[30]
L. brevis NB10	6% maltodextrin + 6% skim milk + 0.25% potassium ascorbate	75.5	This study