Table 5. Properties of L. brevis NB10 powders using mixture of protectants at different use amount

Cryo-protectant agent (w:w)	Use amount (% w/v)	Properties of Freeze-dried powder
		Appearance	Moisture (%)	Survival rate (%)
Man: SM (3:7)	8	Ivory powder, easy to grind into fine particles, low moisture absorbing	2.23	41.28
	10	Ivory powder, easy to grind into fine particles, low moisture absorbing	2.76	55.15
	12	Ivory powder, easy to grind into fine particles, low moisture absorbing	2.51	47.92
Mal: SM (3:7)	8	Ivory powder, easy to grind into fine particles, low moisture absorbing	3.03	39.41
	10	Ivory powder, easy to grind into fine particles, low moisture absorbing	2.70	51.34
	12	Ivory powder, easy to grind into fine particles, low moisture absorbing	2.79	42.06
Mal: SM (5:5)	8	White powder, easy to grind into fine particles, low moisture absorbing	3.12	53.88
	10	White powder, easy to grind into fine particles, low moisture absorbing	2.86	57.66
	12	White powder, easy to grind into fine particles, low moisture absorbing	2.37	65.69