Table 9. Formulations of chewing gum prepared to select the content of excipients

Ingredients	Amount (mg)
	F1	F2	F3	F4	F5	F6	F7	F8	F9
Freeze-dried L. brevis NB10 (*)	100	150	180	200	180	180	180	180	180
Xylitol	100	150	180	200	45	90	180	270	360
Strawberry powder	-	-	-	-	-	-	-	-	-
Glycerol	-	-	-	-	-	-	-	-	-
Talc	-	-	-	-	-	-	-	-	-
Magnesium stearate	-	-	-	-	-	-	-	-	-
Aerosil	24	36	45	48	45	45	45	45	45
HIG04® powder	776	1164	1395	1552	1530	1485	1395	1305	1215
Total weight	1000	1500	1800	2000	1800	1800	1800	1800	1800
Aims	Optimize chewable mass				Adjust gum sweetness

Ingredients	Amount (mg)
	F10	F11	F12	F13	F14	F15	F16	F17	F18	F19	F20
Freeze-dried L. brevis NB10 (*)	180	180	180	180	180	180	180	180	180	180	180
Xylitol	180	180	180	180	180	180	180	180	180	180	180
Strawberry powder	9	18	36	54	36	36	36	36	36	36	36
Glycerol	-	-	-	-	9	18	27	36	27	27	27
Talc	-	-	-	-	-	-	-	-	54	0	27
Magnesium stearate	-	-	-	-	-	-	-	-	0	54	27
Aerosil	45	45	45	45	45	45	45	45	45	45	45
HIG04® powder	1386	1377	1359	1341	1350	1341	1332	1323	1278	1278	1278
Total weight	1800	1800	1800	1800	1800	1800	1800	1800	1800	1800	1800
Aims	Adjust gum flavor				Adjust gum softness				Reduce stickiness

Chewing gums used in the human evaluation test were made from placebo freeze-dried powder which did not contain L. brevis NB10