Table 2. Summary of validation results

Property		QC	TAF	TFV	IS
Selectivity	Analyte (% LLOQ)	-	0.00	0.00
	Internal standard (% IS)	-	0.00	0.00
Carryover	Analyte (% LLOQ)	-	3.40	0.00
	Internal standard (% IS)	-	0.00	0.00	0.00
Linearity	R square (R2)	-	0.9982	0.9962
Intra-day accuracy and precision	Accuracy (%)	LLOQ	110.47	94.18
		LQC	110.82	89.82
		MQC	106.14	113.24
		HQC	100.05	114.03
	Precision, CV (%)	LLOQ	7.55	9.60
		LQC	2.94	5.89
		MQC	2.25	2.00
		HQC	4.69	1.00
Inter-day accuracy and precision	Accuracy (%)	LLOQ	114.51	100.07
		LQC	104.97	87.74
		MQC	101.84	101.82
		HQC	97.11	108.28
	Precision, CV (%)	LLOQ	5.21	11.67
		LQC	8.75	3.98
		MQC	10.48	9.40
		HQC	9.63	5.71
Matrix effect	Precision, CV (%)	LQC	9.57	13.32
		HQC	6.10	2.90
Recovery	Mean	LQC	62.72	94.56	100.10
		MQC	83.85	102.56	87.98
		HQC	72.25	84.44	76.97
	Precision, CV (%)	LQC	5,39	6.75	4.21
		MQC	2,72	3.62	1.79
		HQC	4,31	3.45	2.58
Dilution integrity	Accuracy (%)	-	100.10	103.30
	Precision, CV (%)	-	1.69	4.08
Free-thaw stability	% of nominal concentration	LQC	105.64	91.12
		HQC	102.36	111.97
At room temperature for 6 hours	% of nominal concentration	LQC	95.53	87.71
		HQC	93.68	102.25
In autosampler for 24 hours at 10°C	% of nominal concentration	LQC	102.87	89.06
		HQC	100.33	111.25
Long-term stability (after 60 days)	% of nominal concentration	LQC	101.80	92.79
		HQC	105.95	95.00

Abbreviations: QC = quality control; LLOQ = lower limit of quantification; LQC = low level quality control; MQC = medium level quality control; HQC = high level quality control; IS = internal standard; CV = coefficient of variation