Table 2. Two PCR programs A and B for nested PCR

	Cycles		Temperature		Time		Step
	A	B	A(°C)	B(°C)	A	B
Outer	1	1	94	95	4 min	5 min	Initial denaturation
			94	95	30 sec	30 sec	Denaturation
	36	30	54	56	30 sec	30 sec	Annealing
			72	72	30 sec	30 sec	Extension
	1	1	72	72	10 min	5 min	Final extension
	1	1	4	10	Stored	Stored
Inner	1	1	94	95	5 min	5 min	Initial denaturation
	40	36	94	94	30 sec	30 sec	Denaturation
			56	54	30 sec	30 sec	Annealing
			72	72	30 sec	30 sec	Extension
	1	1	72	72	10 min	5 min	Final extension
	1	1	4	10	Stored	Stored